Martynyuk Neurosciences in Anesthesiology Laboratory
Anatoly Martynyuk, PhD, DSc
Associate Professor of Anesthesiology and Neuroscience
Investigator, McKnight Brain Institute
Mechanisms of long-term effects of neonatal anesthesia
Our research is focused in three major areas:
- The mechanisms of developmental effects of neonatal anesthesia. We test the hypotheses that sevoflurane, isoflurane, propofol and etomidate produce developmental effects via similar mechanisms that involve dysregulation of the limbic-hypothalamic–pituitary–adrenal axis and increase in neuronal activity. Funded by the NIH/NIGMS, and I. Heermann Anesthesia Foundation.
- The contribution of abnormal glutamatergic activity to the etiology of CNS disorders, such as stroke, epileptic seizures, and schizophrenia, as well as drug development for treatments these CNS disorders. We have been studying the concept that antiglutamatergic agents with multisite actions and moderate potency may overcome the side effects of previously tested highly selective antiglutamatergic agents, by producing efficacious neuroprotection while still enabling a level of balanced glutamate receptor activity required for physiological brain functions. Funded by the NIH/NINDS.
- The mechanisms of brain disorders in phenylketonuria (PKU). PKU is the most common disorder of amino acid metabolism (~1/15,000 births) and the most frequent biochemical cause of mental retardation in humans. Based on our experimental results we hypothesize that phenylalanine-impaired glutamatergic activity and diurnal variations in brain phenylalanine levels play an important role in PKU-related brain disorders.
Christoph Seubert, MD, PhD, Associate Professor of Anesthesiology
Clinical aspects of the effects of neonatal anesthesia on developmental outcome
M.D./Ph.D., Medicine/Cardiac – Julius-Maximilians-University,Würzburg,Germany, 1984
M.D./Ph.D., Physiology – Albert-Ludwigs-University, Freiburg, Germany, 1992
Resident/Anesthesia – Ruprecht-Karls-University, Heidelberg, Germany, 1992-1994
Internship/Medicine – Memorial Medical Center, Savannah, Georgia, 1994
Resident/Anesthesia – University of Florida, Gainesville, Florida 1995-1998
Fellowship, Cardiovascular Anesthesia/Research – University of Florida, Gainesville, Florida 1998-2000
Contact Dr. Seubert
Neurohumoral and behavioral effects of general anesthetics in neonatal rats
B.S., University of Florida, Gainesville, FL, 2012
Contact Wanting Zhu
Electroencephalographic effects of general anesthetics in neonatal rats
B.S. University of Florida, Gainesville, FL, 2012
Contact Jesse Willis
Lab Phone: 352-846-1320
Bruno Panzarini, Student
Bruno is involved in investigation of the electroencephalographic effects of general anesthetics in neonatal rats
B.S. University of Florida, Gainesville, FL, 2009-current
Contact Bruno Panzarini
Lab Phone: 352-846-1320
Dianet Puentes, Student
Dianet is involved in investigation of the behavioral effects of general anesthetics in neonatal rats
University of Florida, Gainesville, FL, 2011-current
Contact Dianet Puentes
Lab Phone: 352-846-1320
We welcome applications from undergraduate/graduate students and post-doctoral fellows who would like to join the lab team.
Contact Dr. Martynyuk
Office: MSB M5-526
Seubert CN, Zhu W, Pavlinec C, Gravenstein N, Martynyuk AE. Developmental effects of neonatal isoflurane and sevoflurane exposure in rats. Anesthesiology, 2013 Apr 24. [Epub ahead of print]
Cao W, Pavlinec C, Gravenstein N, Seubert CN, Martynyuk AE. Roles of aldosterone and oxytocin in abnormalities caused by sevoflurane anesthesia in neonatal rats. Anesthesiology, 2012; 117: 791-800.
Edwards DA, Shah HP, Cao W, Gravenstein N, Seubert CN, Martynyuk AE: Bumetanide alleviates epileptogenic and neurotoxic effects of sevoflurane in neonatal rat brain. Anesthesiology 112:567-575, 2010.
Cao W, Shah HP, Glushakov AV, Mecca AP, Shi P, Sumners C, Seubert CN, Martynyuk AE: Efficacy of 3,5-dibromo-L-phenylalanine in rat models of stroke, seizures and sensorimotor gating deficit. Br J Pharmacol 158:2005-2013, 2009.
Martynyuk AE, Ucar DA, Yang DD, Norman WM, Carney PR, Dennis DM, Laipis PJ: Epilepsy in phenylketonuria: a complex dependence on serum phenylalanine levels. Epilepsia 48:1143-1150, 2007.
Glushakov AV, Glushakova O, Varshney M, Bajpai LK, Sumners C, Laipis PJ, Embury JE, Baker SP, Otero DH, Dennis DM, Seubert CN, Martynyuk AE: Long-term changes in glutamatergic synaptic transmission in phenylketonuria. Brain 128(Pt 2):300-307, 2005.
Yarotskyy V, Glushakov AV, Sumners C, Gravenstein N, Dennis DM, Seubert CN, Martynyuk AE: Differential modulation of glutamatergic transmission by 3,5-dibromo-l-phenylalanine. Mol Pharmacol 67:1648-1654, 2005.
Kagiyama T, Glushakov AV, Sumners C, Roose B, Dennis DM, Phillips MI, Ozcan M, Seubert CN, Martynyuk AE: Neuroprotective action of halogenated derivatives of L-phenylalanine. Stroke 35:1192-1196, 2004.
Glushakov AV, Dennis DM, Morey TE, Sumners C, Cucchiara RF, Seubert CN, Martynyuk AE: Specific inhibition of N-methyl-D-aspartate receptor function in rat hippocampal neurons by L-phenylalanine at concentrations observed during phenylketonuria. Mol Psychiatr 7(4):359-367,
Martynyuk AE, Morey TE, Belardinelli L, Dennis DM: Hyperkalemia enhances the effect of adenosine on IK, ADO in rabbit isolated AV nodal myocytes and on AV nodal conduction in Guinea pig isolated heart. Circulation 99:312-318, 1999.